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KMID : 0380020100250030205
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Korean Journal of Biotechnology and Bioengineering
2010 Volume.25 No. 3 p.205 ~ p.214
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Isolation and Purification of Bioactive Materials Using High-Performance Counter-Current Chromatography (HPCCC)
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Jung Dong-Su
Shin Hyun-Jae
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Abstract
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Many successive liquid-liquid extractions occur enabling purification of the crude material to occur. In high performance counter-current chromatography (HPCCC), crude material is partitioned between two immiscible layers of solvent phases. The stationary phase (SP) is retained by hydrodynamic force field effect and the mobile phase (MP) is pumped through the column. Purification occurs because of the different solubility of the components in the liquid mobile and stationary phases. There are many key benefits of liquid stationary phases such as high mass and volume injection loadings, total sample recovery, and easy scale-up. Many researchers showed that predictable scale-up from simple test is feasible with knowledge of the stationary phase retention for the planned process scale run. In this review we review the recent advances in HPCCC research and also describe the key applications such as natural products and synthetics (small or large molecules).
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KEYWORD
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high performance counter-current chromatography (HPCCC), liquid-liquid extraction, stationary phase, mobile phase, sample recovery, scale-up
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